ATCC medium: 1786 Ilyobacter medium
MgCl2 . 6H2O ...................................0.4 g
KCl............................................0.5 g
Na2SO4 .........................................0.1 g
NaCl...........................................1.0 g
NH4Cl ..........................................0.3 g
Na2HPO4 . 12H2O.................................0.7 g
Modified SL-7 Trace Elements (see below).......1.0 ml
0.5% FeCl3 .....................................1.0 ml
Selenite/Tungstate Solution (see below)........1.0 ml
0.1% Resazurin.................................1.0 ml
1.0% CaCl2 . 2H2O ..............................1.0 ml
Crotonic acid..................................1.7 g
Yeast extract..................................1.0 g
Sodium Sulfide Solution (see below)...........10.0 ml
Distilled water................................1.0 L
Adjust to pH 5.5 with NaOH. Boil; cool under 100% N2. Dispense and tube anaerobically under same gas phase. Autoclave at 121C for 15 minutes. Just before use add sodium sulfide solution to a final concentration of 10 mcl/ml medium. Final pH of completed medium should be 6.8-7.2. Modified SL-7
Trace Elements: 1 N HCl............................3.0 ml
ZnCl2 .............................70.0 mg
MnCl2 . 4H2O .....................100.0 mg
H3BO3 .............................60.0 mg
CoCl2 . 6H2O .....................200.0 mg
CuCl2 . 2H2O ......................20.0 mg
NiCl2 . 6H2O ......................20.0 mg
Na2MoO4 . 2H2O.....................40.0 mg
Distilled water....................1.0 L
Selenite/Tungstate Solution: NaOH...............................0.5 g
Na2SeO3 . 5H2O......................3.0 mg
Na2WO4 . 2H2O.......................4.0 mg
Distilled water....................1.0 L
Sodium Sulfide Solution: Na2S . 9H2O ........................3.6 g
Distilled water..................100.0 ml
Bubble with 100% N2; adjust to pH 7.0 with HCl. Tube anaerobically under same gas phase. Autoclave at 121C for 15 minutes.