ATCC medium: 1421 LHET2 medium (ATCC medium 1168) with 0.01% yeast extract or yeast autolysate ATCC Medium 1168 (see below) with 0.01% yeast extract or yeast autolysate
ATCC Medium 1168: Solution A: (NH4)2SO4.........................................................2.0 g
KCl..............................................................0.1 g
K2HPO4 ...........................................................0.5 g
MgSO4 . 7H2O .....................................................0.5 g
Trypticase Soy Broth without Dextrose (BD 211774)................0.1 g
Distilled water................................................500.0 ml
Adjust pH to 2.5-3.0 using 1 N H2SO4. Solution B: Glucose......................1.0 g
Agar (for solid medium).....12.0 g
Distilled water............500.0 ml
Autoclave Solutions A and B separately. Cool to 50C. Add Solution A to