ATCC medium: 542 Peptone-starch-carbonate medium
Peptone......................5.0 g
Yeast extract................5.0 g
K2HPO4 .......................1.0 g
MgSO4 . 7H2O .................0.2 g
Soluble starch..............10.0 g
Agar........................15.0 g
Distilled water............900.0 ml
Augoclave at 121C for 15 minutes. Cool and add aseptically the following filter-sterilized solution:
10% Na2CO3 .................100.0 ml