ATCC Medium: 460 A2E6 Medium
Base Medium
NaCl………………………….……………..23.48 g
MgCl2 x 6H2O…………………………….10.63 g
Na2SO4……………………………………3.92 g
CaCl2 (anhydrous)……….………………1.11 g
KCl……………………………….…………0.66 g
NaHCO3…………………………………...0.19 g
KBr………………………………..………...0.1 g
H3BO3……………………………..……….0.03 g
SrCl2 x 6H2O………………………..……..0.04 g
Metal Mixture (see below)………….……..3.0 ml
FeCl3 x 6H2O…..………………………….0.01 g
Na2 Glycerophosphate……………………0.15 g
(NH4)2SO4…………………...…………….0.05 g
Tris Buffer…………………………………...3.0 g
Vitamin Solution (see below)……….…….1.0 ml
K2HPO4…………………………………….0.01 g
Glucose………………..……………………3.0 g
Glutamic Acid………………………………1.5 g
DI Water…………………………………….1000 ml
Adjust pH to 6.4-6.6. Dispense into appropriate vessel and autoclave at 121?C.
*Dispense 5ml for 16 x 125 mm test tube.
Metal Mixture Na2EDTA………………………………1.0 g
FeCl3 x 6H2O………………………….0.05 g
H3BO3…………………………………1.0 g
MnCl2 x 4H2O………………………...0.15 g
ZnCl2…………………………………..0.01g
CoCl2 x 6H2O…………………………0.005 g
DI Water……………………………….100 ml
Vitamin Solution
Biotin…………………………..………..0.003 g
Thiamine……………………………….1.0 g
DI Water………………………….…….1000 ml