ATCC medium: 1525 Seawater 802 medium
Solution 1:
2X Artificial seawater.................500.0 ml
Filter-sterilize.
Solution 2:
2X Modified ATCC Medium 802 Solution 1 (see below), excluding the Na2HPO4 ............................500.0 ml
Autoclave at 121C for 15 minutes. When cool, aseptically combine Solutions 1 and 2. ATCC Medium 802 Solution 1 Rye grass cerophyll:
Cerophyll*...............................2.5 g
Distilled water..........................1.0 L
Add cerophyll to distilled water and boil for 5 minutes. Add 100 ml distilled water to compensate for evaporation. Filter through Whatman #1 filter paper and add 0.5 g Na2HPO4. Autoclave for 15 minutes at 121C.
*Can be obtained from Ward's Natural Science Establishment, Inc., P.O. Box 92912, Rochester, NY 14692. Dairy Goat Nutrition P.O. Box 22363, Kansas City, MO 64113 distributes Grass Media Culture which is equivalent. Sigma Chemical Company, P.O. Box 14508, St. Louis, MO 63178, sells Cereal Leaf Product no. C-7141 which is similar to Cerophyll.