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生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
ATCC Number：TIB-71
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運(yùn)輸方式：凍存運(yùn)輸
品系：BALB/c
組織來(lái)源：ascites
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
年限：adult
數(shù)量：大量
細(xì)胞形態(tài)：?jiǎn)魏思?xì)胞/巨噬細(xì)胞
規(guī)格：0.5ml
Designations 統(tǒng)一別名 : RAW 264.7
Depositors:WC Raschke
Biosafety Level 生物安全等級(jí) :2
Shipped 提供形式 :frozen
Medium & Serum: See Propagation
Growth Properties:adherent
Organism:Mus musculus
Morphology:monocyte/macrophage
Source:
Strain: BALB/c
Tissue: ascites
Disease: Abelson murine leukemia virus-induced tumor
Cell Type:macrophage; Abelson murine leukemia virus transformed
Cellular Products:lysozyme
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material.
Anyone purchasing ATCC material is ultimately responsible for obtaining the permits.
Please click here for information regarding the specific requirements for shipment to your location.
Applications 用途 :Biological response transfection host
Receptors:complement (C3) [1207 ] Antigen Expression:H-2d
Age:adult
Gender:male
Comments 注釋 :This line was established from a tumor induced by Abelson murine leukemia virus. They are negative for surface immunoglobulin (sIg-), Ia (Ia-) and Thy-1.2 (Thy-1.2) This line does not secrete detectable virus particles and is negative in the XC plaque formation assay.
The cells will pinocytose neutral red and will phagocytose latex beads and zymosan. They are capable of antibody dependent lysis of sheep erythrocytes and tumor cell targets.
LPS or PPD treatment for 2 days stimulates lysis of erythrocytes but not tumor cell targets. Data communicated in Feb. 2007 by Dr Janet W. Hartley, indicates the expression of infectious ecotropic MuLV closely related, if not identical,to the Moloney MuLV helper virus used in the original virus inoculum. The cells also express polytropic MuLV,unsurprisingly based on the mouse passage history of the virus stocks [ PubMed 18177500].
Propagation:ATCC complete growth medium:The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002.
To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere 需氧情況 : air, 95%; carbon dioxide (CO2),5%
Temperature 培養(yǎng)溫度 : 37.0°C
Subculturing:
Protocol: Subcultures are prepared by scraping.
For a 75 cm2 flask, remove all but 10 ml culture medium (adjust amount accordingly for other culture vessels).
Dislodge cells from the flask substrate with a cell scraper; aspirate and add appropriate aliquots of the cell suspension into new culture vessels.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Replace or add medium every 2 to 3 days.
Preservation:
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature 保藏溫度 : liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020
注意事項(xiàng)：
1. 收到細(xì)胞后首先觀察細(xì)胞瓶是否完好，培養(yǎng)液是否有漏液、渾濁等現(xiàn)象，若有上述現(xiàn)象發(fā)生請(qǐng)及時(shí)和我們聯(lián)系。
2. 仔細(xì)閱讀細(xì)胞說(shuō)明書(shū)，了解細(xì)胞相關(guān)信息，如細(xì)胞形態(tài)、所用培養(yǎng)基、血清比例、所需細(xì)胞因子等，確保細(xì)胞培養(yǎng)條件一致。若由于培養(yǎng)條件不一致而導(dǎo)致細(xì)胞出現(xiàn)問(wèn)題，責(zé)任由客戶自行承擔(dān)。
3. 用 75%酒精擦拭細(xì)胞瓶表面，顯微鏡下觀察細(xì)胞狀態(tài)。因運(yùn)輸問(wèn)題貼壁細(xì)胞會(huì)有少量從瓶壁脫落，將細(xì)胞置于培養(yǎng)箱內(nèi)靜置培養(yǎng) 4~6 小時(shí),再取出觀察。此時(shí)多數(shù)細(xì)胞均會(huì)貼壁，若細(xì)胞仍不能貼壁請(qǐng)用臺(tái)盼藍(lán)染色測(cè)定細(xì)胞活力，如果證實(shí)細(xì)胞活力正常，請(qǐng)將細(xì)胞離心后用新鮮培養(yǎng)基再次貼壁培養(yǎng)；如果染色結(jié)果顯示細(xì)胞無(wú)活力，請(qǐng)拍下照片及時(shí)和我們聯(lián)系，信息確認(rèn)后我們?yōu)槟倜赓M(fèi)寄送一次。
4. 靜置細(xì)胞貼壁后，請(qǐng)將細(xì)胞瓶?jī)?nèi)的培養(yǎng)基倒出，留 6~8mL 維持細(xì)胞正常培養(yǎng)，待細(xì)胞匯合度 80%左右時(shí)正常傳代。
5. 請(qǐng)客戶用相同條件的培養(yǎng)基用于細(xì)胞培養(yǎng)。培養(yǎng)瓶?jī)?nèi)多余的培養(yǎng)基可收集備用，細(xì)胞傳代時(shí)可以一定比例和客戶自備的培養(yǎng)基混合，使細(xì)胞逐漸適應(yīng)培養(yǎng)條件。
6. 建議客戶收到細(xì)胞后前 3 天各拍幾張細(xì)胞照片，記錄細(xì)胞狀態(tài)，便于和中國(guó)微生物菌種查詢網(wǎng)（family998.com）技術(shù)部溝通交流。由于運(yùn)輸?shù)脑?，個(gè)別敏感細(xì)胞會(huì)出現(xiàn)不穩(wěn)定的情況，請(qǐng)及時(shí)和我們聯(lián)系，告知細(xì)胞的具體情況，以便我們的技術(shù)人員跟蹤回訪直至問(wèn)題解決。