ATCC Medium: 2831 SDM-79 Medium
SDM-79 (Life Technologies, custom order part # ME090164 P1)……..…….25.5 g
DI Water……………………………………………………………...…….……….1 L
Adjust pH to 7.5 and filter sterilize. Store at 2-8
oC until ready to use. Dispense
SDM-79 medium in 9 ml aliquots into T-25 tissue culture flasks, then add 1.0 ml
of heat-inactivated fetal bovine serum and 150 ?l hemin solution. Final
concentration of hemin should be 7.5 ?g/ml.
Hemin Solution
Hemin …………………………………………………………50.0 mg
DI Water ………………………………………………………100.0 ml
1N NaOH………………………………………………………1.0 ml
Filter sterilize and store at 2-8
oC until ready to use. Hemin solution should not be
kept longer than 30 days.
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