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ATCC Medium: 1611 U4 Medium
Hanks' Balanced Salt Solution, 10X (see below)…………………......4.0 ml
Hartley Digest Broth (HIMEDIA M551).............................................20.0 ml
Fetal Bovine Serum..........................................................................15.0 ml
Yeast Extract Solution (GIBCO 18180)............................................10.0 ml
1% Phenol red..................................................................................0.2 ml
20% Urea..........................................................................................0.25 ml
0.025% MgSO4 x 5H2O……………………………….........................1.0 ml
DI Water to……………………………………….................................100.0 ml
Adjust pH to 6.0-6.2 with HCl and filter-sterilize complete medium.
Hanks' Balanced Salt Solution, 10X
CaCl2 ..........................................1.4 g
KCl...............................................4.0 g
KH2PO4 ......................................0.6 g
MgCl2 x 6H2O .............................1.0 g
MgSO4 x 7H2O ...........................1.0 g
NaCl..............................................80.0 g
Na2HPO4 x 7H2O........................0.9 g
Glucose........................................10.0 g
DI Water to...................................1000.0 ml