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ATCC medium: 462 Prosthecobacter medium
(NH4)2SO4.......................................0.25 g
Glucose........................................0.25 g
Modified Hutner's Basal Salts (see below).....20.0 ml
Na2HPO4 ........................................0.071 g
Distilled water to.............................1.0 L
Autoclave at 121C for 15 minutes.
Modified Hutner's Basal Salts:
Nitrilotriacetic acid.......10.0 g
MgSO4 . 7H2O ................29.7 g
CaCl2 . 2H2O .................3.34 g
Ammonium molybdate...........9.25 mg
FeSO4 . 7H2O ................99.0 mg
Metals "44" (see below).....50.0 ml
Distilled water to...........1.0 L
Dissolve and neutralize the nitrilotriacetic acid with KOH (7.3 g); add
the other ingredients and adjust the pH to 6.6 - 6.8 before bringing the
volume to 1.0 L with distilled water.
Metals "44":
EDTA.........................0.25 g
ZnSO4 . 7H2O .................1.1 g
FeSO4 . 7H2O .................0.5 g
MnSO4 . 7H2O .................0.154 g
CuSO4 . 5H2O .................0.04 g
Co(NO3)2 . 6H2O...............0.025 g
Na2B4O7 . 10H2O ...............0.018 g
Distilled water............100.0 ml
Initially add a few drops of H2SO4 to the distilled water to retard
precipitation.