ATCC Medium: 290 S6 Medium for Thiobacilli
Na2HPO4……………………………..1.2 g
KH2PO4………………………………1.8 g
MgSO4 x 7H2O………………………0.1 g
(NH4)2SO4…………………………...0.1 g
CaCl2 …………………………………0.03 g
FeCl3………………………………….0.02 g
MnSO4………………………………..0.02g
Na2S2O3……………………………..10.0 g
Agar (if needed)……………………...15.0 g
DI Water……………………………….1000 ml
Autoclave at 121?C. Medium will have a slight precipitate. Agitating medium a
little everyday for two consecutive days should diminish the amount of
precipitate.