ATCC medium: 1036 Thiomicrospira medium
NaCl.................................................. 25.0 g
(NH4)2SO4............................................... 1.0 g
MgSO4 . 7H2O ........................................... 1.5 g
CaCl2 .................................................. 0.3 g
K2HPO4 ................................................. 0.5 g
Na2S2O3 . 5H2O .......................................... 8.0 g
Vishniac & Santer Trace Element Solution (see below)... 0.2 ml
Vitamin B12........................................... 15.0 mcg
Distilled water........................................ 1.0 L
Bromcresol purple (0.05% solution)..................... 0.1 ml
Adjust pH to 7.2 and filter-sterilize.
Vishniac and Santer Trace Element Solution:
EDTA........................50.0 g
ZnSO4 . 7H2O ................22.0 g
CaCl2 ........................5.54 g
MnCl2 . 4H2O .................5.06 g
FeSO4 . 7H2O .................4.99 g
(NH4)6Mo7O24 . 4H2O............1.10 g
CuSO4 . 5H2O .................1.57 g
CoCl2 . 6H2O .................1.61 g
Distilled water..............1.0 L
Adjust pH to 6.0 with KOH.