ATCC medium: 2682 Thermoanaerobacter uzonii Medium
K2HPO4 ...................................3.00 g
KH2PO4 ...................................2.00 g
NH4Cl ....................................2.00 g
MgCl2 * 6H2O..............................0.20 g
CaCl2 * 2H2O .............................0.05 g
Glucose..................................5.00 g
Yeast extract............................5.00 g
Resazurin................................0.50 mg
Cysteine-HCl * H2O .......................0.12 g
Na2S * 9H2O ..............................0.12 g
Distilled water..........................1.00 L
Prepare the medium anaerobically using 100% N2.Adjust the pH to 7.0-7.2. Dispense anaerobically 10 mL per
Hungate and autoclave at 121?C for 20 minutes.