ATCC medium: 2681 Caldanaerobacter uzonensis Medium
K2HPO4 ...................................3.0 g
KH2PO4 ...................................2.0 g
NH Cl ....................................2.0 g 4
MgCl2 . 6H20 .............................0.20 g
CaCl2 ....................................0.05 g
Glucose..................................5.0 g
Yeast extract............................5.0 g
Resazurin................................0.50 mg
Cysteine-HCl . H2O .......................0.125 g
Na2S . 9H2O ..............................0.125 g
Distilled water..........................1.0 L
Prepare the medium anaerobically under a steady stream of N2. Adjust the
pH to 7.0 to 7.2. Autoclave at 121?C for 15 minutes.