ATCC medium: 2158 Acidiphilium medium
(NH4)2SO4...........................2.0 g
KCl................................0.1 g
K2HPO4 .............................0.5 g
MgSO4 . 7H2O .......................0.5 g
Glucose (see below)................1.0 g
Yeast extract (see below)..........0.3 g
Distilled water....................1.0 L
Adjust pH to 3.0 with H2SO4. Autoclave glucose and yeast extract separately as 10% and 3% solutions, respectively.
Add to basal medium.