ATCC Medium: 1909 Hutner's Low pH Euglena Medium
KH2PO4………………………………………0.8 g
MgSO4 x 7H2O………………………………1.0 g
CaCO3………………………………………...0.4 g
L-Glutamic acid………………………………10.0g
L-Malic Acid…………………………………..2.0 g
Metals 47 Trace Elements (see below)……0.1 g
FeCl3 x 6H2O………………………………...1.0 mg
Thiamine………………………………………0.2 mg
Vitamin B12…………………………………...0.04 ug
(NH4)2HPO4………………………………….0.4 g
DI Water……………………………………….1000.0 ml
Metals 47 Trace Elements:
Zn(SO4) x 7H2O……………………………..52.8 g
MnS04 x H2O…………………………………24.8 g
Fe(NH4)2(SO4)2 x 6H2O……………………28.0 g
CoSO4 x 7H2O……………………………….4.8 g
CuSO4 x 5H2O……………………………….0.80 g
(NH4)6Mo7O24 x 4H2O……………………..0.36 g
Na3VO4 x 16H2O…………………………….0.37 g
H3BO3…………………………………………1.14 g
Grind salts using a mortar and pestle and store in the cold.
Double Strength Agar:
Agar……………………………………………..80.0 g
DI Water………………………………………...1000.0 ml
Autoclave at 121C.