ATCC medium: 351 Hutner's medium for Euglena
KH2PO4 .........................................0.02 g
Potassium citrate . H2O ........................0.04 g
MgSO4 . 3H2O ...................................0.02 g
Trypticase Peptone (BD 211921).................0.6 g
Liver Concentrate NF #XI Powder* (ICN 100377)..0.2 g
Agar, Noble (BD 214230).......................12.0 g
Distilled water................................1.0 L
Autoclave at 121C for 15 minutes.
*Yeast extract, 0.04 g, thiamine . HCl, 0.04 mg and vitamin B12, 0.05
mcg may be substituted for the Liver Concentrate.