ATCC medium: 725 Liver infusion-sake medium
Infuse 400.0 g fresh liver in 600.0 ml distilled water by boiling for 5-10 minutes.
Filter through Whatman #2 filter paper and add:
Sake....................... 400.0 ml
Agar........................ 15.0 g
Make up volume to 1.0 L. Autoclave 25 minutes at 121C.