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ATCC medium: 1986 V8 juice seawater agar

中國微生物菌種查詢網(wǎng) / 2017-10-11 12:33:08

ATCC medium: 1986 V8 juice seawater agar
Unsalted V8 Juice............................ 200.0 ml 
CaCO3 .......................................... 3.0 g 
Agar.......................................... 15.0 g 
Artificial Seawater (2% NaCl) (see below).... 800.0 ml 
Adjust medium for final pH 7.0. Autoclave at 121C for 15 minutes. 
Artificial Seawater (2% NaCl):  
NaCl........................ 20.0g
MgCl2  . 6H2O ................. 5.38 g 
MgSO4  . 7H2O ................. 6.78 g 
KCl.......................... 0.72 g 
NaHCO3 ....................... 0.2 g
CaCl2  . 2H2O ................. 1.4 g 
Distilled water.............. 1.0 L

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