ATCC Medium: 2882 Ferrovum myxofaciens Medium
*Basal salts concentrate (see below)……………………..20 ml
*Trace elements solution (see below)…………………….1 ml
DI Water……………………………………………………..1000 ml
Adjust pH to 2.1 and autoclave at 121C.
Filter sterilized ferrous sulfate solution (pH 2.0) is added to give a final ferrous iron concentration of 5mM. Basal salts concentrate
MgSO4?7H2O……………………………………….25 g
(NH4)2SO4…………………………………………..22.5 g
Na2SO4?10H2O……………………………………..7.5 g
KH2PO4……………………………………………..2.5 g
KCl…………………………………………………..2.5 g
Ca(NO3)2·4H2O…………………………………….0.7 g
DI Water…………………………………………….1000 ml
Trace elements solution ZnSO4·7H20……………………………………….10 g
CuSO4·5H2O………………………………………1 g
MnSO4·4H2O………………………………………1 g
CoSO4·7H2O……………………………………….1g
Cr2(SO4)3·15H2O…………………………………..0.5 g
H3BO3……………………………………………….0.6 g
Na2MoO4·2H2O…………………………………….0.5 g
NiSO4·6H2O………………………………………..1 g
Na2SeO4·10H2O…………………………………..1 g
Na2WO4?2H2O……………………………………..0.1 g
NaVO3………………………………………………0.1 g
DI Water……………………………………………1000 ml
*Both of these can be supplied with the culture, if requested. Note that any general-purpose trace elements mixture will probably be appropriate.