ATCC medium: 2363 Brain heart infusion, 1/10 strength
Brain Heart Infusion (BD 237500)......... 3.7 g
Agar, if required....................... 15.0 g
Distilled water.......................... 1.0 L
Prepare infusion according to manufacturer’s directions. Adjust pH to 7.4 +/- 0.2. Autoclave at 121C for 15 minutes