ATCC medium: 890 Tryptone-glucose medium
Tryptone (BD 211705)........ 20.0 g
Glucose...................... 5.0 g
KH2PO4 ....................... 0.2 g
K2HPO4 ........................ 0.35 g
MgCl2 6H2O................. 0.1 g
CaCl2 . 2H2O ................. 0.07 g
Distilled water.............. 1.0 L
Add 50 mcg biotin and 200 mcg thiamine per liter. Autoclave at 121C for 15 minutes.