ATCC medium: 355 Crithidia medium
Trypticase Peptone (BD 211921)................. 6.0 g
Yeast extract.................................. 1.0 g
Liver Concentrate NF #XI Powder (ICN 100377)... 0.1 g
Sucrose....................................... 15.0 g
Hemin Solution (see below)..................... 5.0 ml
Distilled water................................ 1.0 L
Adjust the pH to 7.8 before autoclaving. Use 5 ml of medium per 16 X 125 mm screw-capped test tube. Slight hemin precipitate is normal following autoclaving. Autoclave at 121C for 15 minutes.
Hemin Solution:........ 2.5 ml
Triethanolamine (TEA) Hemin....................... 25.0 mg
Distilled water.............. 2.5 ml