ATCC medium: 1716 Corn steep liquor medium
(NH4)2SO4...........................3.3 g
CaCl2 .............................11.0 mg
KH2PO4 ...........................990.0 mg
MgSO4 ............................120.0 mg
FeSO4 ..............................2.0 mg
Na2MoO4 ............................0.2 mg
ZnSO4 ............................970.0 mcg
MnSO4 ............................910.0 mcg
CuSO4 .............................32.0 mcg
Vitamin Mix (see below)...........10.0 ml
Glucose...........................40.0 g
Corn steep liquor*................50.0 ml
Antifoam (Dow Corning B)...........0.1 ml
Distilled water to.................1.0 L
*Supernatant fraction after centrifugation. Adjust medium for final pH 5.0 +/- 0.2. Autoclave at 121C for 15 min.
Vitamin Mix: Inositol.........................200.0 mg
Niacin............................40.0 mg
Pyridoxine hydrochloride..........40.0 mg
Thiamine . HCl....................40.0 mg
Calcium D-(+)-pantothenate........40.0 mg
Riboflavin........................20.0 mg
p-Aminobenzoic acid...............20.0 mg
Folic acid.........................0.2 mg
Biotin.............................0.2 mg
Distilled water....................1.0 L