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ATCC medium: 1957 Geobacter medium

中國(guó)微生物菌種查詢網(wǎng) / 2017-05-04 09:12:47

ATCC medium: 1957 Geobacter medium

 NH4Cl ....................................1.5 g 

NaH2PO4 ..................................0.6 g 

KCl......................................0.1 g 

NaHCO3 ...................................2.5 g 

Sodium acetate...........................0.82 g 

Sodium fumarate (filter-sterilized)......8.0 g 

Wolfe's Vitamin Solution (see below)....10.0 ml 

Modified Wolfe's Minerals (see below)...10.0 ml 

Distilled water to.......................1.0 L 

Prepare and dispense medium without fumarate anaerobically under an atmosphere of 80% N2, 20% CO2. Autoclave at 121C for 15 minutes. Add fumarate from a filter-sterilized, nitrogen-sparged stock solution prior to inoculation. Final pH of medium should be approximately 6.8. Wolfe's Vitamin Solution: Available from ATCC as a sterile ready-to-use liquid (Vitamin 

Supplement, catalog no. MD-VS). 

Biotin.......................2.0 mg 

Folic acid...................2.0 mg 

Pyridoxine hydrochloride....10.0 mg 

Thiamine . HCl...............5.0 mg

 Riboflavin...................5.0 mg 

Nicotinic acid...............5.0 mg 

Calcium D-(+)-pantothenate...5.0 mg 

Vitamin B12..................0.1 mg 

p-Aminobenzoic acid..........5.0 mg 

Thioctic acid................5.0 mg 

Distilled water..............1.0 L 

Modified Wolfe's Minerals: 

Na2SeO3 .................................10.0 mg 

NiCl2 . 6H2O ............................10.0 mg

 Na2WO4 . 2H2O............................10.0 mg

 Wolfe's Mineral Solution (see below).....1.0 L

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