ATCC菌種 陰道毛滴蟲 百歐博偉生物
小楊 / 2019-12-06 09:48:31
陰道毛滴蟲（Trichomonas vaginalis, Donne 1837）是寄生在人體陰道和泌尿道的鞭毛蟲，主要引起滴蟲性陰道炎和尿道炎，是以性傳播為主的一種傳染病。
基本信息
平臺編號：bio-48621
規(guī)格：frozen
拉丁屬名：Trichomonas vaginalis Donne
中文名：陰道毛滴蟲
釋 義： 寄生在人體陰道和泌尿道的鞭毛蟲
詳情介紹
Trichomonas vaginalis Donne 拉丁名
ATCC 30184 編號
Strain Designations 菌株別名 123414
本菌形態(tài)：陰道毛滴蟲的生活史僅有滋養(yǎng)體階段而無包囊階段?；铙w呈無色透明，有折光性，體態(tài)多變，活動力強(qiáng)。固定染色后呈梨形，體長7～23μm，前端有一個泡狀核，核上緣有5顆排列成環(huán)狀的基體，由此發(fā)出5根鞭毛：4根前鞭毛，1根后鞭毛。1根軸柱，纖細(xì)透明，縱貫蟲體，自后端伸出體外。體外側(cè)前1/2處，有一波動膜，其外緣與向后延伸的后鞭毛相連。蟲體借助鞭毛擺動前進(jìn)，以波動膜的波動作旋轉(zhuǎn)式運(yùn)動。胞質(zhì)內(nèi)有深染的顆粒，為該蟲特有的氫化酶體（hydrogenosome）。
Application 用途
Sexually Transmitted Disease Research
Biosafety Level 安全等級 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation Vaginal swab; Nashville, TN; 1964
Product Format 提供形式 frozen
Storage Conditions 培養(yǎng)條件Frozen 冷凍物 Cultures:-70°C for 1 week; liquid N2 vapor for long term storage
Freeze-dried 凍干物 Cultures:
2-8°C
Live Cultures:
See Protocols section for handling information
Type Strain no
Medium 培養(yǎng)基 ATCC Medium 2154: LYI Entamoeba medium
ATCC Medium 361: Modified TYM basal medium (ATCC medium 358) with pH adjusted to 6.0 and 0.2-0.5 ml of heat-inactivated horse serum added per tube before use
Growth Conditions 生長條件Temperature 培養(yǎng)溫度 : 35°C
Atmosphere 需氧情況 : Anaerobic
Culture System: Axenic; pH 6.0
Cryopreservation Harvest and Preservation
Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min. The cells grown in a medium containing agar are concentrated by centrifugation, a solid pellet does not form. The soft pellet is resuspended to desired cell concentration with agar-free supernatant.
Adjust the concentration of cells to 2 x 106 to 2 x 107/mL in fresh medium.
While cells are centrifuging prepare a 10% (v) solution of sterile DMSO in fresh medium.
Add 1.0 mL of DMSO to an ice cold 20 x 150 mm screw-capped test tube.
Place the tube on ice and allow the DMSO to solidify (~5 min) and then add 9.0 mL of ice cold medium.
Invert several times to dissolve the DMSO.
Allow to warm to room temperature.
Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 106 to 107 cells/mL and 5% (v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should no less than 15 min and no longer than 30 min.
Dispense in 0.5 mL aliquots into 1.0 mL to 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
Place the vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion. At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.)
The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -55°C.To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just above the surface of the frozen material. Do not agitate the vial.
Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate a 16 x 125 mm screw-capped test tube containing either 9 mL of ATCC medium 361 (completed with serum) or 13 mL ATCC Medium 2154 adjusted to pH 6.0.
Incubate the culture at 35°C with the cap screwed on tightly (tube should be vertical for medium 361 or on a 15° horizontal slant for medium 2154).
Name of Depositor 寄存人 R Samuels
Year of Origin 1964
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