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Name:

Caniella muris

DSM No.:

110323, Proposed type strain

Strain designation:

CLA-SR-94

Isolated from:

caecal content; wild mouse

Country:

Germany

Aachen

Nagoya Protocol Restrictions:

There are NO known Nagoya Protocol restrictions for this strain.

History:

<- T. Clavel, RWTH Aachen; CLA-SR-94

Genbank accession numbers:

16S rRNA gene: OM658553

Cultivation conditions:

Medium 339 Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). For solid media 5% horse blood., 37°C

For reactivation use the complete content of the ampoule for inoculation.

Please follow special instructions: 'Cultivation of Anaerobes'

Complete DSMZ Media List

Summary and

additional information:

<- T. Clavel, RWTH Aachen; CLA-SR-94. Caecal content; wild mouse. Germany, Aachen. Sequence accession no. 16S rRNA gene: OM658553.poposed name: Caniella muris

. (Medium 339 Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). For solid media 5% horse blood., incubation time: 2-3 days, gas phase: 80%N2, 20%CO2, pH 7, 37°C, anaerobic). For reactivation use the complete content of the ampoule for inoculation.

Risk group:

2 (provisional classification by DSMZ)