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"Clostridium ragsdalei"

DSM No.:

15248, Proposed type strain

Strain designation:

P11

Isolated from:

duck pond sediment

Country:

United States of America

Oklahoma

Date of sampling:

1998

Nagoya Protocol Restrictions:

There are NO known Nagoya Protocol restrictions for this strain.

History:

<- R. S. Tanner, University of Oklahoma, Department of Botany and Microbiology, Norman, USA; P11 <- R. Laopaiboon; {1999}

Genbank accession numbers:

16S rRNA gene: DQ020022

draft genome: LROS00000000

Cultivation conditions:

Medium 339a with strain-specific modifications, 35°C

or

Medium 104c with strain-specific modifications, 35°C

 

Cells of this strain lyse rapidly; thus, transfer to fresh medium immediately upon receipt of an active culture. Active cultures may be shipped on slant agar to maintain viability during transportation. For inoculation in liquid medium, add 0.5 mL of medium to the tube with slant agar, suspend the cells, and transfer the suspension to the tube with liquid medium. Please ensure that anoxic and sterile conditions are maintained during the transfer of the culture.

 

Incubation time: 2-3 days

 

Please follow special instructions: 'Cultivation of Anaerobes'

 

Complete DSMZ Media List

Summary and

additional information:

<- R. S. Tanner, University of Oklahoma, Department of Botany and Microbiology, Norman, USA; P11 <- R. Laopaiboon; {1999}. Duck pond sediment. United States of America, Oklahoma. Sequence accession no. 16S rRNA gene: DQ020022, draft genome: LROS00000000. Strain-specific literature (38870). (Medium 339a with strain-specific modifications, incubation time: 2-3 days, 35°C, anaerobic or Medium 104c with strain-specific modifications, incubation time: 2-3 days, 35°C, anaerobic). Cells of this strain lyse rapidly; thus, transfer to fresh medium immediately upon receipt of an active culture. Active cultures may be shipped on slant agar to maintain viability during transportation. For inoculation in liquid medium, add 0.5 mL of medium to the tube with slant agar, suspend the cells, and transfer the suspension to the tube with liquid medium. Please ensure that anoxic and sterile conditions are maintained during the transfer of the culture.