Cell line:MFE-319
DSMZ no.:ACC 423
Species:human (Homo sapiens)
Cell type:endometrium adenocarcinoma
Origin:established from the primary adenosquamous endometrium carcinoma (grading G1-G2) of an 81-year-old woman in 1992; described in the literature to form poorly differentiated tumors in nude mice
Reference(s):14587
Biosafety level:1
Permissions and restrictions:A
DSMZ Cell Culture Data:
Morphology:epithelial-like adherent cells growing as monolayer; image; image
Medium:40% RPMI 1640 + 40% MEM (with Earle's salts) + 20% h.i. FBS
Subculture:seed out at ca. 1-2 x 106 cells/25 cm2; split confluent culture 1:2 to 1:3 every 5 days using trypsin/EDTA (cells are difficult to detach from the flask; thoroughly washing with PBS and incubation with trypsin/EDTA of 15 min or longer at 37 °C is recommended)
Incubation:at 37 °C with 5% CO2
Doubling time:ca. 70-90 hours
Harvest:cell harvest of ca. 15-20 x 106 cells/80 cm2
Storage:frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:originally negative in DAPI, microbiological culture, RNA hybridization, and continuously by PCR assays
Immunology:To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:human hypodiploid karyotype with 4% polyploidy - 45(43-46)<2n>X, -X, t(13;18)(q14.3-21;q11-12) - resembles published karyotype
Viruses:ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
