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 Nitrospira defluvii

DSM 118082


BACTERIA
How to read the following data (Example)

Name:
"Nitrospira defluvii" Spieck et al. 2006

DSM No.:
118082

Strain designation:
A17

Other collection no.or WDCM no.:
LMG 27402,NCIMB 14950

Isolated from:
activated sludge

Country:
GermanyWastewater treatment plant Dradenau Hamburg

Date of sampling:
1997-11-11

Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.

Cultivation conditions:
Medium 756d use 0.02 g/l NaNO2, aerobic growth in darkness, no shaking, 28°CMedium/media recipe(s) required for preparing 756d:-Medium 756Use 100 - 150 ml medium in 300 ml Erlenmeyer flasks with metal caps. The medium has to be prepared 2-3 days before inoculation. The final pH should be 7.4-7.6. Inoculate 1 ml per flask and incubate the culture in the dark without stirring. It might take several weeks until the nitrite is consumed. Use a spot test for presence of nitrite on filter paper (e.g., MQuant Nitrite Test, Sigmaldrich 1.10007.0001).Complete DSMZ Media List

Summary andadditional information:
Activated sludge. Germany, Wastewater treatment plant Dradenau Hamburg. Strain-specific literature (32162, 41036, 41037, 41038). (Medium 756d use 0.02 g/l NaNO2, incubation time: more than 14 days, gas phase: 100% air, 28°C, aerobic, aerobic growth in darkness, no shaking). Use 100 - 150 ml medium in 300 ml Erlenmeyer flasks with metal caps. The medium has to be prepared 2-3 days before inoculation. The final pH should be 7.4-7.6. Inoculate 1 ml per flask and incubate the culture in the dark without stirring. It might take several weeks until the nitrite is consumed. Use a spot test for presence of nitrite on filter paper (e.g., MQuant Nitrite Test, Sigmaldrich 1.10007.0001).

Literature:
32162,41036,41037,41038,41047

Risk group:
1 (provisional classification by DSMZ)