Escherichia coli
DSM 115365
BACTERIA
How to read the following data (Example)
Name:
Escherichia coli (Migula 1895) Castellani and Chalmers 1919
DSM No.:
115365
Strain designation:
Nissle 1917 (T7); EcNT7
Isolated from:
Laboratory culture
Country:
Germany
Date of sampling:
13.10.2019
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
History:
<- Kerstin Fiege and Nicole Frankenberg-Dinkel, Technical University Kaiserslautern; Nissle 1917 (T7); EcNT7
Cultivation conditions:
Medium 381 , 37°C
Complete DSMZ Media List
Summary and
additional information:
<- Kerstin Fiege and Nicole Frankenberg-Dinkel, Technical University Kaiserslautern; Nissle 1917 (T7); EcNT7. Until 2023 preserved as Escherichia coli. Laboratory culture. Germany. Strain carries chromosomal integration of T7 polymerase under control of lacUV5 promoter; use for the heterologous expression of heme cofactor; therefore, strain is suitable for expression of genes under control of T7 promoter; the strain is unique in that it is able to take up heme intrinsically from culture media so that heterologously produced proteins can be loaded better with heme cofactor. The plasmid used for gene integration of T7 polymerase into the genome (pUC-T7-FRT-Kan) was removed from chromosome by FLP-FRT-site technique, strain carries neither the plasmid nor antibiotic resistances, vector was excised from the genome by lambda recombination, strain does not contain any remains of the plasmid. The T7 polymerase is necessary for gene expression under control of the T7 promoter, used in expression vectors like the pET or pDuet.
Please note, very important! E. coli strain IPC-ntb2 mentioned in S. Ferrari et al., Int. J. Food Microbiol 388 (2023) and in ISO 10272:2017/AMD 2023 is NOT DSM 115365. IPC-ntb2 is DSM 116329!
. (Medium 381, 37°C)
Risk group:
1 (classification according to German TRBA)
DSM115365
