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 Species:Escherichia coli

Group:K12

Strain designation:MG1655 SEGA032

Accession number:LMBP 12868

Depositor:A.Ehrmann1 and Prof.Dr M.Nørholm1.It was constructed by C.Bayer1,A.Ehrmann1 and M.Rennig1.

1 The Novo Nordisk Foundation Center for Biosustainability,Technical University of Denmark,Horsholm,Denmark

Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).

Other culture collection numbers:SEGA 032

Medium:LB-Lennox

Selection marker:tetracycline(10µg/ml)

Temperature:37°C

Genotype:F-λ-ilvG-rfb-50 rph-1 glmS-pstS::Ptrc-low-PelB-sfgfp-tetA(Source:http://openwetware.org/wiki/E._coli_genotypes#MG1655+Bayer et al.,2021)

SEGA sequence:p12868.gb

Phenotype:tetR

Properties:The strain was created based on Escherichia coli K12 MG1655,by integrating the SEGA landing pad between the glmS and pstS loci.Counter-selection is posssible through sensitivity to NiCl2(50µM).

Further information:This host strain is intended for simplistic bacterial genome engineering,using the SEGA genome architecture.

Original reference:Bayer et al.,Nat.Commun.12(2021),5876[DOI:10.1038/s41467-021-26155-5]

Restricted use:-BCCM MTA

-The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department.

Refer in your Materials and Methods:

Escherichia coli K12 MG1655 SEGA032(LMBP 12868)is available at BCCM/GeneCorner.It was deposited by A.Ehrmann and Prof.Dr M.Nørholm and was published in Bayer et al.,2021.