Candidatus Nitrospira inopinata
DSM 105286
BACTERIA
How to read the following data (Example)
Name:
"Candidatus Nitrospira inopinata"
DSM No.:
105286
Strain designation:
ENR4
Other collection no.
or WDCM no.:
JCM 31988
Isolated from:
biofilm of oil exploration well pipe
Country:
Russian Federation
oil exploration well pipe (43° 22' 45'' N, 43° 43' 26.1'' E)
Date of sampling:
2011-04-01
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
History:
<- C. Sedlacek, Department of Microbial Ecology, University of Vienna (Nitrospira inopinata)<- E. Lebedeva, Winogradsky Institute of Microbiology, University Vienna, Austria
Genbank accession numbers:
complete genome: LN885086
BioSample: SAMEA3560316
BioProject: PRJEB10818
Cultivation conditions:
Medium 1690 , 42°C
Medium/media recipe(s) required for preparing 1690:
- Medium 320
- Medium 385
Does not tolerate shaking. Growth takes place without visible increase in turbidity, hence proliferation of cells has to be checked using phase contrast microscopy. Nitrite/Nitrate production is a reliable indication of growth and can be checked with Griess-Ilosvay's reagent. However, it is more accurate to measure consumption of ammonium using the OPA reagent as described by Holmes et al. 1999 (26775). Based on the slow growth and low cell number the production and delivery might take more than 2 months.
Incubation time: more than 14 days
Complete DSMZ Media List
Summary and
additional information:
<- C. Sedlacek, Department of Microbial Ecology, University of Vienna (Nitrospira inopinata)<- E. Lebedeva, Winogradsky Institute of Microbiology, University Vienna, Austria. Biofilm of oil exploration well pipe. Russian Federation, oil exploration well pipe (43° 22' 45'' N, 43° 43' 26.1'' E). Sequence accession no. complete genome: LN885086, BioSample: SAMEA3560316, BioProject: PRJEB10818. ''Nitrospira inopinata'', ''Candidatus Nitrospira inopinata''. Chemolithoautotrophic.
growth on guanidine, guanidinase (CUQ66148)
(41244). Complete nitrification; "comammox" bacteria (27655, 27656). Strain-specific literature (27656). (Medium 1690, incubation time: more than 4 weeks, 42°C). Does not tolerate shaking. Growth takes place without visible increase in turbidity, hence proliferation of cells has to be checked using phase contrast microscopy. Nitrite/Nitrate production is a reliable indication of growth and can be checked with Griess-Ilosvay's reagent. However, it is more accurate to measure consumption of ammonium using the OPA reagent as described by Holmes et al. 1999 (26775). Based on the slow growth and low cell number the production and delivery might take more than 2 months.
Literature:
27655, 27656, 41244, 41019
Risk group:
1 (provisional classification by DSMZ)
DSM105286
