Species:
Escherichia phage M13 (Inovirus M13)
Taxonomy:
Order: Tubulavirales
Family: Inoviridae
Subfamily:
Genus: Inovirus
Isolate:
Escherichia phage M13
DSM No.:
13976
Strain designation:
PC-V3465
History:
<- NCCB
Isolated from:
sewage
Geographic origin:
country of origin unknown
Date of sampling:
before 11.01.2001
Nagoya Protocol Restrictions:
There are NO known Nagoya protocol restrictions for this strain.
Genomics:
ssDNA
Morphology:
flexible filament
Plaques description:
very small, turbid
Image:
Single plaques on agar plate.
Creator: B. Henze, Leibniz Institute DSMZ.
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Leibniz-Institut DSMZ
Further information:
male specific (4409). Cells are not killed by infection. Single-stranded DNA, filamentous (4410). As an alternative host, DSM 11539 can be used. Host propagation on F+, F' or Hfr strains, F pili used as receptor. Used in Expt. 49 in "Sourcebook of Experiments for the Teaching of Microbiology", eds. S.B. Primrose and A.C. Wardlaw, Academic Press (1982). A series of M13 derivatives has been constructed as cloning vectors for DNA sequencing.
Literature:
4408, 4409, 4407, 4410, 23821
Risk group:
1
Phage propagation
Host bacterium:
Escherichia coli (Migula 1895) Castellani and Chalmers 1919
DSM-5695
We strongly recommend to use only DSM-5695 for propagation.
Cultivation conditions:
Medium 544 plus 2.0 mg of streptomycin per l, 37°C
Complete DSMZ Media List
Phage propagation:
Supply, storage and propagation of phages
Revitalisation of Vacuum-Dried Phages
Escherichia phage M13 (Inovirus M13)
Taxonomy:
Order: Tubulavirales
Family: Inoviridae
Subfamily:
Genus: Inovirus
Isolate:
Escherichia phage M13
DSM No.:
13976
Strain designation:
PC-V3465
History:
<- NCCB
Isolated from:
sewage
Geographic origin:
country of origin unknown
Date of sampling:
before 11.01.2001
Nagoya Protocol Restrictions:
There are NO known Nagoya protocol restrictions for this strain.
Genomics:
ssDNA
Morphology:
flexible filament
Plaques description:
very small, turbid
Image:
Single plaques on agar plate.Creator: B. Henze, Leibniz Institute DSMZ.
Leibniz-Institut DSMZFurther information:
male specific (4409). Cells are not killed by infection. Single-stranded DNA, filamentous (4410). As an alternative host, DSM 11539 can be used. Host propagation on F+, F' or Hfr strains, F pili used as receptor. Used in Expt. 49 in "Sourcebook of Experiments for the Teaching of Microbiology", eds. S.B. Primrose and A.C. Wardlaw, Academic Press (1982). A series of M13 derivatives has been constructed as cloning vectors for DNA sequencing.
Literature:
4408, 4409, 4407, 4410, 23821
Risk group:
1
Phage propagation
Host bacterium:
Escherichia coli (Migula 1895) Castellani and Chalmers 1919
DSM-5695
We strongly recommend to use only DSM-5695 for propagation.
Cultivation conditions:
Medium 544 plus 2.0 mg of streptomycin per l, 37°C
Complete DSMZ Media List
Phage propagation:
Supply, storage and propagation of phages
Revitalisation of Vacuum-Dried Phages
DSM13976
