Nitrososphaera viennensis
DSM 26422
ARCHAEA
How to read the following data (Example)
Name:
Nitrososphaera viennensis Stieglmeier et al. 2014
DSM No.:
26422, Type strain
Other collection no.
or WDCM no.:
EN76,JCM 19564
Isolated from:
garden soil
Country:
Austria
Vienna, 9th, district
Date of sampling:
2008
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
History:
<- M Melcher and C. Schleper, University Vienna, Fac. of Life Scs, Vienna, Austria; {2021} <- C. Schleper, University Vienna, Fac. of Life Scs, Vienna, Austria; EN76 {2016} <- M. Stieglmeier and C. Schleper;
Genbank accession numbers:
16S rRNA gene: FR773157
amoA gene: FR773159
amoB gene: FR773160
complete genome: CP007536
Cultivation conditions:
Medium 1630 , 40°C
Medium/media recipe(s) required for preparing 1630:
- Medium 141
- Medium 722
or
Medium 1630c , (this medium is recommended for the routine cultivation of the strain), 40°C
Medium/media recipe(s) required for preparing 1630c:
- Medium 141
- Medium 320
- Medium 385
Growth takes place without visible increase in turbidity, hence proliferation of cells has to be checked using phase contrast microscopy. Nitrite production is a reliable indication of growth and can be checked with Griess-Ilosvay's reagent. However, it is more accurate to measure consumption of ammounium using the OPA reagent as described by Holmes et al. 1999 (26775)
Incubation time: more than 14 days
Complete DSMZ Media List
Summary and
additional information:
<- C. Schleper, Univ. Vienna, Fac. of Life Scs, Austria <- M. Stieglmeier and C. Schleper {2010}. Garden soil; Austria, Vienna, 9th, district (19786). Type strain. Taxonomy/description (19762). Sequence accession no. 16S rRNA gene: FR773157, amoA gene: FR773159, amoB gene: FR773160, complete genome: CP007536. Oxidizes ammonia to nitrite with oxygen as electron acceptor. Strain-specific literature (26775). (Medium 1554, 42°C). Growth takes place without visible increase in turbidity, hence proliferation of cells has to be checked using phase contrast microscopy. Nitrite production is a reliable indication of growth and can be checked with Griess-Ilosvay's reagent. However, it is more accurate to measure consumption of ammounium using the OPA reagent as described by Holmes et al. 1999 (26775).
Literature:
19762, 19786, 26775
Risk group:
1 (classification according to German TRBA)
DSM26422
DSM 26422
ARCHAEA
How to read the following data (Example)
Name:
Nitrososphaera viennensis Stieglmeier et al. 2014
DSM No.:
26422, Type strain
Other collection no.
or WDCM no.:
EN76,JCM 19564
Isolated from:
garden soil
Country:
Austria
Vienna, 9th, district
Date of sampling:
2008
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
History:
<- M Melcher and C. Schleper, University Vienna, Fac. of Life Scs, Vienna, Austria; {2021} <- C. Schleper, University Vienna, Fac. of Life Scs, Vienna, Austria; EN76 {2016} <- M. Stieglmeier and C. Schleper;
Genbank accession numbers:
16S rRNA gene: FR773157
amoA gene: FR773159
amoB gene: FR773160
complete genome: CP007536
Cultivation conditions:
Medium 1630 , 40°C
Medium/media recipe(s) required for preparing 1630:
- Medium 141
- Medium 722
or
Medium 1630c , (this medium is recommended for the routine cultivation of the strain), 40°C
Medium/media recipe(s) required for preparing 1630c:
- Medium 141
- Medium 320
- Medium 385
Growth takes place without visible increase in turbidity, hence proliferation of cells has to be checked using phase contrast microscopy. Nitrite production is a reliable indication of growth and can be checked with Griess-Ilosvay's reagent. However, it is more accurate to measure consumption of ammounium using the OPA reagent as described by Holmes et al. 1999 (26775)
Incubation time: more than 14 days
Complete DSMZ Media List
Summary and
additional information:
<- C. Schleper, Univ. Vienna, Fac. of Life Scs, Austria <- M. Stieglmeier and C. Schleper {2010}. Garden soil; Austria, Vienna, 9th, district (19786). Type strain. Taxonomy/description (19762). Sequence accession no. 16S rRNA gene: FR773157, amoA gene: FR773159, amoB gene: FR773160, complete genome: CP007536. Oxidizes ammonia to nitrite with oxygen as electron acceptor. Strain-specific literature (26775). (Medium 1554, 42°C). Growth takes place without visible increase in turbidity, hence proliferation of cells has to be checked using phase contrast microscopy. Nitrite production is a reliable indication of growth and can be checked with Griess-Ilosvay's reagent. However, it is more accurate to measure consumption of ammounium using the OPA reagent as described by Holmes et al. 1999 (26775).
Literature:
19762, 19786, 26775
Risk group:
1 (classification according to German TRBA)
DSM26422
