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　　Species:Escherichia coli

　　Group:K12

　　Strain designation:S26

　　Accession number:LMBP 4977

　　Depositor:Prof.Dr E.Remaut1 2

　　1 Department for Molecular Biomedical Research,VIB,Ghent,Belgium

　　2 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium

　　It was obtained from Dr A.Garen3

　　3 Department of Molecular Biology and Biophysics,Yale University,USA

　　Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).

　　Other culture collection numbers:CGSC 4620

　　Medium:LB-Lennox

　　Selection marker:/

　　Temperature:37°C

　　Genotype:Hfr garB10 fhuA22 phoA4(Am)ompF627(T2R)fadL701(T2R)relA1 pitA10 spoT1 rrnB-2 mcrB1 creC510(Source:CGSC 4620)

　　Phenotype:phosphatase negative

　　Properties:This strain carries the S26 amber mutation(TAG)in the phosphatase gene.This strain is the parental strain of a series of four isogenic strains differing only in their suppressor activity(see accession numbers LMBP 4977 up to and including LMBP 4980).

　　Further information:-

　　Original reference:Garen et al.,J.Mol.Biol.14(1965),167-178[PMID:5327650]

　　Related reference:Remaut et al.,J.Mol.Biol.71(1972),243-261[PMID:4564480]

　　Restricted use:-BCCM MTA

　　Refer in your Materials and Methods:

　　Escherichia coli K12 S26(LMBP 4977)is available at BCCM/GeneCorner.It was deposited by Prof.Dr E.Remaut and was published in Garen et al.,1965.