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平臺(tái)編號(hào)：bio-127938
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國(guó)際編號(hào)：LMBP 671
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拉丁屬名： Escherichia coli
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規(guī)格：/
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用途：BCCM原裝進(jìn)口
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu) 說(shuō)明書(shū)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
SG4121
Species:Escherichia coli
Group:K12
Strain designation:SG4121
Accession number:LMBP 671
Depositor:Prof.E.Remaut1 2
1 Department for Molecular Biomedical Research,VIB,Ghent,Belgium
2 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium
It was obtained from Dr S.Gottesman3
3 Laboratory of Molecular Biology,NCI,NIH,Bethesda,USA
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:LB-Lennox
Selection marker:/
Temperature:37°C
Genotype:lon-100Δ(gal-pgl)324 lac thi rpsL recA hsdR
Phenotype:StrR Lon-rK-mK+non-mucoid
Properties:-
Further information:The Lon protease plays a major role in the proteolytic machinery of E.coli.Lon mutants are highly pleioptropic and produce mucoid colonies that are difficult to handle.The(gal-pgl)deletion eliminates mucoidy and restores normal colony morphology.
Lon mutants may be useful in stabilizing heterologous proteins expressed in E.coli(see e.g.Remaut et al.,1983).
In addition,this strain is K12 restriction negative/modification positive,and RecA deficient.
Original reference:-
Related reference:Remaut et al.,Nucleic Acids Res.11(1983),4677-4688[PMID:6308555]
Keidel et al.,Eur.J.Biochem.204(1992),1141-1148[PMID:1312934]
Restricted use:-BCCM MTA
Refer in your Materials and Methods:
Escherichia coli K12 SG4121(LMBP 671)is available at BCCM/GeneCorner.It was deposited by Prof.E.Remaut.
LMBP671
