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SM10(λpir)
    SM10(λpir)
  • 平臺(tái)編號(hào):bio-127933
  • 國(guó)際編號(hào):LMBP 3889
  • 拉丁屬名: Escherichia coli
  • 規(guī)格:/
  • 用途:BCCM原裝進(jìn)口
  • 服務(wù)費(fèi)用:
    加載中……
  • 訂購(gòu) 說(shuō)明書(shū)
  • 注意事項(xiàng):僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫(kù)為準(zhǔn))

  Species:Escherichia coli

  Group:K12

  Strain designation:SM10(λpir)

  Accession number:LMBP 3889

  Depositor:Dr J.J.Mekalanos1

  1 Department of Microbiology and Molecular Genetics,Harvard Medical School,Boston,USA

  Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).

  Medium:LB-Lennox

  Selection marker:kanamycin(50μg/ml)

  Temperature:37°C

  Genotype:thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu Kmλpir

  Phenotype:KanR

  Properties:Donor strain(mobilizing strain)carrying the transfer genes of the broad host range IncP type plasmid RP4 integrated in its chromosome.Useful for mobilizing mobRP4 plasmids.

  Further information:The presence ofλpir allows the replication of a defective R6K suicide plasmid and derivatives thereof.

  Original reference:-

  Related reference:Simon et al.,Biotechnology 1(1983),784-791

  Restricted use:-BCCM MTA

  Refer in your Materials and Methods:

  Escherichia coli K12 SM10(λpir)(LMBP 3889)is available at BCCM/GeneCorner.It was deposited by Dr J.J.Mekalanos.

LMBP3889

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