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　　Species:Escherichia coli

　　Group:K12

　　Strain designation:MC1061

　　Accession number:LMBP 9560

　　Depositor:Prof.Dr E.Remaut1 2

　　1 Department for Molecular Biomedical Research,VIB,Ghent,Belgium

　　2 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium

　　Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).

　　Other culture collection numbers:CGSC 6649

　　LMBP 00472

　　Medium:LB-Lennox

　　Selection marker:streptomycin(25μg/ml)

　　Temperature:37°C

　　Genotype:F-Δ(araA-leu)7697[araD139]B/rΔ(codB-lacI)3 galK16 galE15(GalS)λ-e14-mcrA0 relA1 rpsL150 spoT1 mcrB1 hsdR2(Source:CGSC 6649)

　　Phenotype:StrR rK-mK+ZeoR

　　Properties:Useful host for primary transformation.Transforms very well by the CaCl2 method(10exp7/μg).There is no Type I restriction;incoming DNA receives the E.coli K modification.As this strain is deleted for the lacI repressor gene,it is not a suitable host for plasmids carrying the lac promoter or derivatives thereof,such as tac,trc,N25/O2,...promoters.In the absence of repression,continuous transcription from the lac promoter is likely to result in plasmid instability.The zeocin resistance is most probably related to the presence of a functional recA gene(recA+)(personal communication John Wertz,director E.coli Genetick Stock Center).

　　Further information:-

　　Original reference:Casadaban et al.,J.Mol.Biol.138(1980),179-207[PMID:6997493]

　　Related reference:Brigéet al.,Biochem.J.394(2006),335-344[PMID:16293111]

　　Restricted use:-BCCM MTA

　　Refer in your Materials and Methods:

　　Escherichia coli K12 MC1061(LMBP 9560)is available at BCCM/GeneCorner.It was deposited by Prof.Dr E.Remaut and was published in Casadaban et al.,1980.