Source:Mouse
Type:Embryonic stem(ES)cells
Cell line designation:G4 ROSALUC B12
Other designation:G4 ROSALUC ES
Accession number:LMBP 10507CB
Depositor:Dr T.Hochepied1 2,Dr L.Haenebalcke1 2 and Prof.Dr J.Haigh1 2
1 Inflammation Research Center,VIB,Ghent,Belgium
2 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:?500 ml bottle Knockout DMEM(Invitrogen,cat.no.10829-018)
?90 ml serum
?6 ml penicillin/streptomycin 100X
?6 ml beta-mercaptoethanol 100X
?6 ml non-essential amino acids 100X
?6 ml glutamine 100X
?recombinant mouse LIF 2000 units/ml
Selection marker:puromycin(1μg/ml)
Temperature:37°C
Cultivation remarks:Recovery:startup in a 6-well with inactivated feeders after washing.
Genotype:-
Phenotype:-
Properties:-
Further information:-
Original reference:Haenebalcke et al.,Stem Cell Rev.6(2013),774-785[PMID:23877658]
Restricted use:-VIB/MSH/BCCM MTA
-Commercial,for-profit entities require a use license from VIB/MT SINAI prior to the release of the material.
Cultivation remarks:Recovery:startup in a 6-well with inactivated feeders after washing.
Refer in your Materials and Methods:
G4 ROSALUC mouse ES cells(LMBP 10507CB)is available at BCCM/GeneCorner.It was deposited by Dr T.Hochepied,Dr L.Haenebalcke and Prof.Dr J.Haigh and was published in Haenebalcke et al.,2013.
LMBP10507CB
Type:Embryonic stem(ES)cells
Cell line designation:G4 ROSALUC B12
Other designation:G4 ROSALUC ES
Accession number:LMBP 10507CB
Depositor:Dr T.Hochepied1 2,Dr L.Haenebalcke1 2 and Prof.Dr J.Haigh1 2
1 Inflammation Research Center,VIB,Ghent,Belgium
2 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:?500 ml bottle Knockout DMEM(Invitrogen,cat.no.10829-018)
?90 ml serum
?6 ml penicillin/streptomycin 100X
?6 ml beta-mercaptoethanol 100X
?6 ml non-essential amino acids 100X
?6 ml glutamine 100X
?recombinant mouse LIF 2000 units/ml
Selection marker:puromycin(1μg/ml)
Temperature:37°C
Cultivation remarks:Recovery:startup in a 6-well with inactivated feeders after washing.
Genotype:-
Phenotype:-
Properties:-
Further information:-
Original reference:Haenebalcke et al.,Stem Cell Rev.6(2013),774-785[PMID:23877658]
Restricted use:-VIB/MSH/BCCM MTA
-Commercial,for-profit entities require a use license from VIB/MT SINAI prior to the release of the material.
Cultivation remarks:Recovery:startup in a 6-well with inactivated feeders after washing.
Refer in your Materials and Methods:
G4 ROSALUC mouse ES cells(LMBP 10507CB)is available at BCCM/GeneCorner.It was deposited by Dr T.Hochepied,Dr L.Haenebalcke and Prof.Dr J.Haigh and was published in Haenebalcke et al.,2013.
LMBP10507CB
