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　　Species:Candida albicans

　　Group:

　　Strain designation:Tet-Off-RT2

　　Accession number:LMBP 10530

　　Depositor:Prof.Dr K.Ganesan1.It was constructed by Dr S.Bijlani1.

　　1 CSIR Institute of Microbial Technology,Chandigarh,India

　　Containment level:This strain has been assigned the containment level Class 2 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).

　　Medium:YPD(1%bacto-yeast extract,2%bacto-peptone and 2%dextrose)

　　Selection marker:nourseothricine(200μg/ml)

　　Temperature:30°C

　　Genotype:HAH2-pTET-TUP1/HIS1-pTET-TUP1

　　Phenotype:NtcR

　　Properties:The strain contains the C.albicans TUP1 gene under the control of a TET promoter,and a Tet-Off transactivator under the control of the C.albicans RP10 promoter,integrated at the RP10 locus.

　　Further information:The strain is a derivative of the C.albicans Tet-Off-R strain(LMBP 10528)which is a derivative of C.albicans SN95.

　　Original reference:Bijlani et al.,Curr.Genet.64(2018),303-316[PMID:28597304][DOI:10.1007/s00294-017-0720-9]

　　Restricted use:-BCCM MTA

　　-The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded namely UGent-DBMB and VIB-IRC.

　　Refer in your Materials and Methods:

　　Candida albicans Tet-Off-RT2(LMBP 10530)is available at BCCM/GeneCorner.It was deposited by Prof.Dr K.Ganesan and was published in Bijlani et al.,2018.