Name:
"Coleofasciculus sp."
DSM No.:
101195
Other collection no.
or WDCM no.:
CCY0002
Isolated from:
Intertidal habitat (marine); sediment
Country:
Netherlands
North sea, barrier island Schiermonnikoog
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
History:
<- L. J. Stal, Royal Netherlands Institute for Sea Research (NIOZ); {2015} <- U. I. A. Wollenzien, Royal Netherlands Institute for Sea Research (NIOZ); CCY0002 {2000}
Genbank accession numbers:
16S ribosomal gene RNA, partial sequence: GQ402017.1
16S + 23S partial sequence, intergenic spacer: OR297946
Cultivation conditions:
Medium 1677 , 17°C
At the DSMZ, the cyanobacterial cultures are usually cultivated without agitation in 25 cm2 plastic tissue culture flasks with filter screw caps. To subculture Coleofasciculus strains, scrape off the adhering cell material with a plastic Pasteur pipette and crush it by pipetting up and down several times. Alternatively, use a disposable pipette tip to dissect the cell material. Use 2-3 ml of the shredded biomass to inoculate 15 ml of fresh medium in a new tissue culture flask. Light cycle: 16 hours of illumination at 650 lux and 8 hours of darkness. Transfer interval of 12 weeks.
Complete DSMZ Media List
Risk group:
1 (classification according to German TRBA)
DSM101195
