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J26 拉丁名

(ATCC? CRL-1802?) 編號(hào)

Organism Mus musculus, mouse 

Product Format 提供形式 frozen 

Morphology fibroblast Culture Properties adherent 

Biosafety Level 安全等級(jí) 1 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Age 100 days 

Gender male 

Strain 菌株別名 C3H/An 

Applications 用途 This line was derived from the L-M(TK-) cell line by transformation with human genomic DNA and a plasmid containing the Herpes simplex virus thymidine kinase gene and then selecting for transformants positive for beta 2 microglobulin. The cells can be used as recipients for the the transfer of individual HLA genes. Tested and found negative for ectromelia virus (mousepox). 

Derivation This line was derived from the L-M(TK-) cell line by transformation with human genomic DNA and a plasmid containing the Herpes simplex virus thymidine kinase gene and then selecting for transformants positive for beta 2 microglobulin. 

Clinical Data male 

Genes Expressed human beta-2-microglobulin (beta 2 microglobulin) 

Cellular Products human beta-2-microglobulin (beta 2 microglobulin) 

Comments 注釋This line was derived from the L-M(TK-) cell line by transformation with human genomic DNA and a plasmid containing the Herpes simplex virus thymidine kinase gene and then selecting for transformants positive for beta 2 microglobulin. The cells can be used as recipients for the the transfer of individual HLA genes. Tested and found negative for ectromelia virus (mousepox). 

Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.03 mM hypoxanthine, 0.001 mM amethopterin and 0.02 mM thymidine, 90%; fetal bovine serum, 10% 

Subculturing Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: 2 to 3 times per week Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. 

Cryopreservation Culture medium, 95%; DMSO, 5% 

Culture Conditions 培養(yǎng)條件

Temperature 培養(yǎng)溫度 : 37.0°C

Name of Depositor 寄存人 P Kavathas 

Deposited As Mus musculus 

References 參考文獻(xiàn) Kavathas P, Herzenberg LA. Stable transformation of mouse L cells for human membrane T-cell differentiation antigens, HLA and beta 2-microglobulin: selection by fluorescence-activated cell sorting. Proc. Natl. Acad. Sci. USA 80: 524-528, 1983. PubMed: 6188154