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生長狀態(tài)：懸浮生長
ATCC Number：CRL-2340?
相關(guān)疾?。簩Ч馨?br /> 細胞形態(tài)：上皮樣
運輸方式：凍存運輸
器官來源：乳房
數(shù)量：大量
年限：TNM stage IIIA, grade 2
組織來源：duct
是否是腫瘤細胞：1
物種來源：人
規(guī)格：100 ul Designations: HCC2157
Depositors: ?AF Gazdar, AK Virmani
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension, multicell aggregates, Cells in suspension show an inclination to form duct-like structures and gland-like structures.
Organism: Homo sapiens
Morphology:epithelial


Source: Organ: mammary gland; breast
Tissue: duct
Tumor Stage: TNM stage IIIA, grade 2
Disease: primary ductal carcinoma
Cellular Products:Epithelial glycoprotein 2 [EGP2]; cytokeratin 19
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935.
Applications:HCC2157 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.
The cells are negative for expression of estrogen receptor (ER -), but positive for expression of progesterone receptor (PR +).
The cells are positive for expression of Her2-neu and for expression of p53.
The cells form ducts and morula-like structures (hollow spherical structures lined with a single layer of cells).
Receptors:estrogen receptor, negative
progesterone receptor, positive
Oncogene:her2/neu +, p53 +
DNA Profile (STR):Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 11
D5S818: 13
D7S820: 10,12
THO1: 8,9
TPOX: 9,11
vWA: 14,18
Cytogenetic Analysis:Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22%
Age: 48 years adult
Gender: female
Ethnicity: Black
Comments:This cell line was initiated on 3/4/96 and took 8 months to establish.
The tumor was classified as TNM stage IIIA, grade 2, ductal carcinoma with 1 out of 9 lymph node metastasis.
The cells form ducts and morula-like structures (hollow spherical structures lined with a single layer of cells).
The cells are positive for expression of Her2-neu and for expression of p53.
HCC2157 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.
The cells are negative for expression of estrogen receptor (ER -), but positive for expression of progesterone receptor (PR +).
An EBV transformed lymphoblastoid cell line (HCC2157 BL) from the same patient is available as ATCC CRL-2341 .
Propagation: ATCC complete growth medium: ACL-4 medium supplemented with 10% FBS
The base medium for this cell line is ATCC-formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:

1. 0.02 mg/ml insulin
2. 0.01 mg/ml transferrin
3. 25 nM sodium selenite
4. 50 nM Hydrocortisone
5. 1 ng/ml Epidermal Growth Factor (do not filter)
6. 0.01 mM ethanolamine
7. 0.01 mM phosphorylethanolamine
8. 100 pM triiodothyronine
9. 0.5% (w/v) bovine serum albumin
10. 10 mM HEPES
11. 0.5 mM sodium pyruvate
12. 2mM L-glutamine(for final conc. of 4.5 mM)
13. 10% FBS

Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Cultures grow as floating aggregates of large irregular cell clusters.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
recommended serum:ATCC 30-2020
Medium additive:ATCC 30-2214
derived from same individual:ATCC CRL-2341
References: 38266: Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771