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RD (ATCC? CCL-136?)Organism Homo sapiens, human Tissue muscle Product Format frozen Morphology spindle cells and large multinucleated cells Culture Properties adherent Biosafety Level 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Disease rhabdomyosarcoma Age 7 years Gender female Ethnicity Caucasian Applications This cell line can be used for the detection of viruses. Storage Conditions liquid nitrogen vapor phaseKaryotype unstable within a hyperdiploid bimodal stemline number of 49 and 50. Twenty-two cells had chromosome associations, 15 cells microchromosomes, 2 cells had fragments, 2 cells with breaks, 2 cells with achromatic gaps and 1 cell with a secondary constriction Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. Images Clinical Data 7 years Caucasian female Genes Expressed myoglobin; myosin ATPase Cellular Products myoglobin myosin ATPase Virus Susceptibility Human poliovirus 1 Vesicular stomatitis, Glasgow (Indiana) Vesicular stomatitis, Orsay (Indiana) Herpes simplex virus Vaccinia virus Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Volumes used in this protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 3 to 4 days Cryopreservation Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase Culture Conditions Temperature: 37°CSTR Profile Amelogenin: X CSF1PO: 10,11 D13S317: 13 D16S539: 10,11 D5S818: 11 D7S820: 8,12 THO1: 9.3 TPOX: 9 vWA: 18 Isoenzymes G6PD, B Name of Depositor RM McAllister Deposited As Homo sapiens References American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992. McAllister RM, et al. Cultivation in vitro of cells derived from a human rhabdomyosarcoma. Cancer 24: 520-526, 1969. PubMed: 4241949 Chen TR, et al. Cell identity resolved. Nature 340: 106, 1989. PubMed: 2739733 Stratton MR, et al. Misidentified cell. Nature 337: 311-312, 1989. PubMed: 2911385 Gershwin ME, et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. PubMed: 857033 Hahon N, Cooke KO. Primary virus-cell interactions in the immunofluorescence assay of Venezuelan equine encephalomyelitis virus. J. Virol. 1: 326, 1967. PubMed: 4918236 Guerin E, et al. Stromelysin-3 induction and interstitial collagenase repression by retinoic acid. J. Biol. Chem. 272: 11088-11095, 1997. PubMed: 9111003 Jeffers M, et al. Degradation of the Met tyrosine kinase receptor by the ubiquitin-proteasome pathway. Mol. Cell. Biol. 17: 799-808, 1997. PubMed: 9001234 Massuda ES, et al. Regulated expression of the diphtheria toxin A chain by a tumor-specific chimeric transcription factor results in selective toxicity for alveolar rhabdomyosarcoma cells. Proc. Natl. Acad. Sci. USA 94: 14701-14706, 1997. PubMed: 9405676 Tiger CF, et al. Presence of laminin alpha5 chain and lack laminin alpha1 chain during human muscle development and in muscular dystrophies. J. Biol. Chem. 272: 28590-28595, 1997. PubMed: 9353324 Sabbioni S, et al. GOK: a gene at 11p15 involved in rhabdomysarcoma and rhabdoid tumor development. Cancer Res. 57: 4493-4497, 1997. PubMed: 9377559 Xiao GH, et al. The Tuberous Sclerosis 2Gene Product, Tuberin, Functions as a Rab5 GTPase Activating Protein (GAP) in Modulating Endocytosis. J. Biol. Chem. 272: 6097-6100, 1997. PubMed: 9045618 Cross References Nucleotide (GenBank) : U09279 Human type XIX collagen (COL19A1) mRNA, partial cds.