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MEL-14 [MEL-14.D54] 拉丁名

(ATCC? HB-132?) 統(tǒng)一編號

Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) 

Cell Type hybridoma: B lymphocyte 

Product Format 提供形式 frozen 

Morphology lymphoblast 

Culture Properties suspension 

Biosafety Level 生物安全等級 1 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Disease Lymphoma 

Applications 用途 Animals were immunized with 38C-13 cells (a B cell lymphoma cell line derived from a C3H/eb mouse). 

The antibody is specific for a 90000 dalton lymphocyte surface receptor for high endothelial venule determinants in peripheral lymph nodes. 

The antibody binds to a to a determinant present in a peptide containing the 13 C terminal residues of ubiquitin. 

Tested and found negative for ectromelia virus (mousepox).

Derivation Animals were immunized with 38C-13 cells (a B cell lymphoma cell line derived from a C3H/eb mouse). 

Spleen cells were fused with P3X63Ag8.653 myeloma cells. 

Genes Expressed immunoglobulin; monoclonal antibody; against lymphocyte surface receptor for endothelium (murine equivalent to human L-selectin, CD62L, Leu-8) 

Cellular Products immunoglobulin; monoclonal antibody; against lymphocyte surface receptor for endothelium (murine equivalent to human L-selectin, CD62L, Leu-8) 

Comments 注釋 Animals were immunized with 38C-13 cells (a B cell lymphoma cell line derived from a C3H/eb mouse). 

Spleen cells were fused with P3X63Ag8.653 myeloma cells. 

The antibody is specific for a 90000 dalton lymphocyte surface receptor for high endothelial venule determinants in peripheral lymph nodes. This receptor appears to be required for lymphocyte homing into lymph nodes in vivo. 

The antibody binds to a to a determinant present in a peptide containing the 13 C terminal residues of ubiquitin. 

It does not bind to native ubiquitin or to ubiquitinated proteins. 

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. 

To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. 

Subculturing Medium Renewal: Every 2 to 3 days 

Cultures can be maintained by addition or replacement of fresh medium. 

Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. 

Cryopreservation Freeze medium: complete growth medium, 95%; DMSO, 5% 

Isotype IgG2a

Name of Depositor 寄存人 EC Butcher 

Deposited As rat (B cell); mouse (myeloma) 

References 參考文獻 Gallatin WM, et al. A cell-surface molecule involved in organ-specific homing of lymphocytes. Nature 304: 30-34, 1983. PubMed: 6866086 

Siegelman M, et al. Cell surface molecule associated with lymphocyte homing is a ubiquitinated branched-chain glycoprotein. Science 231: 823-829, 1986. PubMed: 3003913 

Wilson ME, et al. Local suppression of IFN-gamma in hepatic granulomas correlates with tissue-specific replication of Leishmania chagasi. J. Immunol. 156: 2231-2239, 1996. PubMed: 8690913