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D15 

(ATCC? 95487?) 統(tǒng)一編號 

Designations 別名 D15 

Gen Bank Number L10820 

Species Homo sapiens, human 

Vector Construct size (kb): 4.349999904632568 

DESCRIPTION OF VECTOR COMPONENT: 

Name of vector: pBluescript SK- Intact vector 

size: 2.960 

Type of vector: phagemid 

Vector end: EcoRI 

Vector end: XhoI 

Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI 

Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ',promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, <- promoter: lac, <- primer site: M13 reverse, <- replicon: pMB1 marker(s): ampR, <- 

Cross references: Insert DNA: cDNA DESCRIPTION OF INSERT COMPONENT: 

Genome: Homo sapiens Gene symbol: FPR1 Gene name: formyl peptide receptor 1 Contains complete coding sequence?: Y Chromosome: 19; Localization: 19 q13.4 Tissue: HL-60 cell line Disease: promyelocytic leukemia 

Type of DNA: cDNA 

Insert 5' end: Modification: EcoRI linker 

Insert 3' end: Modification: XhoI linker 

Insert size (kb): 1.35 

Cross references: DNA Seq. Acc.: L10820 

Nucleotides 1-1350 of the insert correspond to nucleotides 525-552, 5370-6599 of L10820. 

Insert lengths(kb): 1.350000023841858 

Tissue: HL-60 cell line promyelocytic leukemia 

Disease: promyelocytic leukemia 

Gene product: formyl peptide receptor 1 [FPR1] 

Insert Size (kb) 1.350 

Biosafety Level 生物安全等級 1 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) 

Comments Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; XhoI--4.3; EcoRI/XhoI--2.9, 1.4; BamHI--4.3; PstI--3.2, 1.1. 

The insert includes the following restriction sites (approximate kb from the 5' end): NcoI--0.25; PvuII--1.0; PstI--1.14; SacI--1.18. 

Lacks exon 2 in the 5' untranslated region that is present in some alternatively spliced transcripts. 

Begins within exon 1 and contains the complete exon 3. References Murphy PM, et al. Sequence and organization of the human N-formyl peptide receptor-encoding gene. Gene 133: 285-290, 1993. PubMed: 8224916 

Murphy PM, McDermott D. Functional expression of the human formyl peptide receptor in Xenopus oocytes requires a complementary human factor. J. Biol. Chem. 266: 12560-12567, 1991. PubMed: 1712023 

Murphy PM, et al. A structural homologue of the N-formyl peptide receptor. Characterization and chromosome mapping of a peptide chemoattractant receptor family. J. Biol. Chem. 267: 7637-7643, 1992. PubMed: 1373134