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CCD-34Lu 拉丁名

(ATCC® CRL-1491™) 統(tǒng)一編號

Organism Homo sapiens, human

Tissue lung

Cell Type fibroblast

Product Format 提供形式 frozen

Morphology fibroblast

Culture Properties adherent

Biosafety Level 生物安全等級 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal

Age 2.5 weeks

Gender female

Ethnicity Black

Applications 用途 The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility).

A normal skin line derived from the same patient is available as ATCC CRL-1497

Derivation The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility).

A normal skin line derived from the same patient is available as ATCC CRL-1497

Clinical Data The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility). A normal skin line derived from the same patient is available as ATCC CRL-1497 female Black 2.5 weeks

Comments 注釋 The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility).

A normal skin line derived from the same patient is available as ATCC CRL-1497

Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003.

To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium 培養(yǎng)基 Renewal: Twice per week Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 5 to 10 minutes.

Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days.

Culture Conditions 培養(yǎng)條件 

Temperature 培養(yǎng)物 : 37.0℃

STR Profile

Amelogenin: X CSF1PO: 11,13

D13S317: 9,13

D16S539: 8,13

D5S818: 12

D7S820: 10

THO1: 7,9

TPOX: 11 vWA: 15,16

Name of Depositor  寄存人 GP Maxwell