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平臺(tái)編號(hào)：bio-80898
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國(guó)際編號(hào)：ATCC CRL-6546
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細(xì)胞信息： OA3.Ts
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規(guī)格：frozen
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用途：ATCC原裝進(jìn)口
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu) 說(shuō)明書(shū)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
OA3.Ts
OA3.Ts 拉丁名
(ATCC® CRL-6546™) 統(tǒng)一別名
Organism Ovis aries, sheep
Tissue testis
Product Format 提供形式 frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 生物安全等級(jí) 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease normal
Age fetus
Gender male
Storage Conditions 保藏條件 liquid nitrogen vapor phase
Clinical Data male
Comments 注釋 Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
1.Remove and discard culture medium.
2.Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
4.Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting
5.Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
6.Incubate cultures at 37C.
Cryopreservation Freeze medium: complete growth medium, 95%; DMSO, 5%
Culture Conditions 培養(yǎng)條件
Temperature 培養(yǎng)溫度 : 37.0℃
Atmosphere 需氧情況 : air, 95%; carbon dioxide (CO2), 5%
