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WU E-4 [1506 A1.4]

(ATCC? CRL-2247?)

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Cell Type hybridoma: B lymphocyte

Product Format frozen

Morphology lymphoblast

Culture Properties suspension

Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications The antibody binds to an epitope in amino acids 126 through 191 on human ApoE.

Derivation Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Genes Expressed immunoglobulin; monoclonal antibody; against human apolipoprotein E (ApoE)

Cellular Products immunoglobulin; monoclonal antibody; against human apolipoprotein E (ApoE)

Tumorigenic Yes

Effects Yes, forms ascites in pristane primed BALB/c mice

Comments Animals were immunized with the major protein peak (G-200 fractionation) of delipidated human high density lipoprotein (HDL). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody binds to an epitope in amino acids 126 through 191 on human ApoE. It can block ApoE mediated binding of lipoproteins to the the low density lipoprotein (LDL) receptor.

Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.1 mM non-essential amino acids and 1 mM sodium pyruvate, 85%; horse serum, 10%; fetal bovine serum, 5%

Subculturing Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium.

Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Cryopreservation Culture medium, 95%; DMSO, 5%

Isotype IgG1