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CV-1

(ATCC? CCL-70?)

Organism Cercopithecus aethiops

Tissue kidney

Product Format frozen

Morphology fibroblast Culture

Properties adherent

Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Normal

Age 141 days

Gender male Applications efficacy testing； transfection host viruscide testing；

Storage Conditions liquid nitrogen vapor phase

Karyotype This is a pseudodiploid, male African green monkey cell line. The modal chromosome number was 60, occurring in 48% of cells, and the rate of polyploidy was at 4.4%. Only a few markers were found., Of these M1, a probable deleted N11, was found in all cells examined; M3 of unknown origin was in some cells; and the remaining 2 to 3 others of unknown origins were found only once., N11 was uniformly single copied, and N16 was also single copied in most cells. Both X and Y chromosomes were also detected in every cell.

Derivation The CV-1 cell line was derived from the kidney of a male adult African green monkey by F.C. Jensen, et al. in March, 1964 for use in Rous sarcoma virus transformation studies. Clinical Data adult African green monkey male

Virus Susceptibility Human poliovirus 1

Herpes simplex virus

Eastern equine encephalitis virus ,

Eastern equine encephalitis virus

encephalitis virus ,

 encephalitis virus

California encephalitis virus , Melao virus

SV40 virus

Comments This cell line is a suitable host for transfection, especially by SV40 vectors.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.,

1，Remove and discard culture medium.

2，Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3，Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37℃ to facilitate dispersal.

4，Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

5，Resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.

6，Place culture vessels in incubators at 37℃.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation Culture medium, 95%; DMSO, 5%

Culture Conditions

Temperature: 37℃

Name of Depositor FC Jensen

Year of Origin 1964

References Jensen FC, et al. Infection of human and simian tissue cultures with Rous Sarcoma Virus. Proc. Natl. Acad. Sci. USA 52: 53-59, 1964. PubMed: 14192657 Kit S, et al. Enhanced thymidine kinase activity following infection of green monkey kidney cells by simian adenoviruses, simian papovavirus SV40, and an adenovirus-SV40 "hybrid". Virology 27: 453-457, 1965. PubMed: 4285109 Deleersnyder V, et al. Formation of native hepatitis C virus glycoprotein complexes. J. Virol. 71: 697-704, 1997. PubMed: 8985401

Rhoades KL, et al. Synergistic up-regulation of the myeloid-specific promoter for the macrophage colony-stimulating factor receptor by AML1 and the t(8;21) fusion protein may contribute to leukenogenesis. Proc. Natl. Acad. Sci. USA 93: 11895-11900, 1996. PubMed: 8876234

Halford WP, et al. Mechanisms of herpes simplex virus type 1 reactivation. J. Virol. 70: 5051-5060, 1996. PubMed: 8764012

Wu TT, et al. Evidence that two latency-associated transcripts of herpes simplex virus type 1 are nonlinear. J. Virol. 70: 5962-5967, 1996. PubMed: 8709218

Berson JF, et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 8709256

Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260

Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128 Planelles V, et al. Vpr-induced cell cycle arrest is conserved among primate lentiviruses. J. Virol. 70: 2516-2524, 1996. PubMed: 8642681

Hill JM, et al. In vivo epinephrine reactivation of ocular herpes simplex virus type 1 in the rabbit is correlated to a 370-base-pair region located between the promoter and the 5' end of the 2.0-kilobase latency-associated transcript. J. Virol. 70: 7270-7274, 1996. PubMed: 8794381

Dubuisson J, Rice CM. Hepatitis C virus glycoprotein folding: disulfide bond formation and association with calnexin. J. Virol. 70: 778-786, 1996. PubMed: 8551615

Biardi L, Krisans SK. Compartmentalization of cholesterol biosynthesis. J. Biol. Chem. 271: 1784-1788, 1996. PubMed: 8576183

Pei L. Identification of a negative glucocorticoid response element in the rat type 1 vasoactive intestinal polypeptide receptor gene. J. Biol. Chem. 271: 20879-20884, 1996. PubMed: 8702844 Hocking AM, et al. Eukaryotic expression of recombinant biglycan. J. Biol. Chem. 271: 19571-19577, 1996. PubMed: 8702651

Standard Test Method for Determining the Virus-Eliminating Effectiveness of Liquid Hygienic Handwash and Handrub Agents Using the Fingerpads of Adult Volunteers. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1838-02.

Standard Test Method for Evaluation of Handwashing Formulation for Virus-Eliminating Activity using the Entire Hand. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2011-99.

Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2197-02.

Cross References Nucleotide (GenBank) : U83324 Cercopithecus aethiops CC chemokine receptor-5 (CCR5) gene, clone 1, complete cds.

Nucleotide (GenBank) : U83325 Cercopithecus aethiops CC chemokine receptor-5 (CCR5) gene, clone 2, complete cds.